Lightsheet Microscopy

The lighsheet microscope is located in G.069

Light sheet microscopy uses a focused sheet of light to illuminate one plane of a sample at a time whilst images are captured to the side. This allows fast, high-resolution 3D imaging with minimal photodamage since only the observed plane is exposed to light. It is used for capturing dynamic 3D imaging of living samples such as organoids and large cleared specimens at unmatched speed and sensitivity.

System properties:

  • Gentle, fast imaging for live or cleared samples
  • Temperature and gas incubation for live imaging (e.g. organoids, embryos)
  • 3 chamber sizes for samples up to ~2 cm (e.g. mouse brain)
  • 4-laser system (405, 488, 561, 638 nm) with dual sCMOS pco.edge cameras
Zeiss Lightsheet 7
Zeiss Lightsheet 7
  • Illumination objectives: 5x/0.1 foc and 10x/0.2 foc
  • Water detection objectives: 5x, 10x, 20x
  • Cleared detection objectives: 2.5x, 5x, 10x, 20x (RI 1.35-1.58)
  • Dedicated preparation room with bench space, Stemi microscope, refractometer and heat block
  • ARIVIS VISION4D analysis station
  • Virtual Reality: ARIVIS VR and Oculus Rift enable VR visualisation of samples. Ideal for public engagement work.
Small sample chamber for live imaging
Small sample chamber for live imaging
In Situ Hybridisation of a chick embryo Wing to Look at Bones (magenta) and Nerve (yellow)
In Situ Hybridisation of a chick embryo Wing to Look at Bones (magenta) and Nerve (yellow)

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