The NARF has gene editing facilities for the chicken, a variety of ubiquitous and gene-specific reporter lines. HTML Fertile eggs and chicks are available for research use upon request, with a requirement for a material transfer agreement for some lines to meet our restrictions relating to animal experiments or the source of the lines.Resources for gene editingGenetically altered linesDescriptionFacilityAvailabilityiCaspase9 surrogate hosts Further informationThe iCaspase9 line can be used to create new genetically altered poultry lines. In the iCaspase9 surrogate host line, both male and female germ cell lineages can be chemically ablated.Sire Dam Surrogate mating results in 100% transmission of introduced primordial germ cells in the first generation of offspring.Conventionaland SPFAvailable Reporter linesGenetically altered lines DescriptionFacilityAvailabilityACTN (actin and nuclear markers)Further information Reporter line. Life-actin-Venus, Histone H2B-cherry. Ubiquitous expression of life-actin-VenusFP and nuclear-cherryFP ConventionalAvailableRoslin Green(Cytoplasmic GFP) Further information Reporter line. Cells from GFP chickens ubiquitously express cytoplasmic eGFP, allowing descendants to be traced after grafting or in cell culture. ConventionalAvailableMembrane GFPFurther informationReporter line. Membrane-localised eGFP.Useful for identifying individual cell boundaries when imaging. ConventionalAvailableFlamingo (TdTomato)Further information Reporter line. Cells from this line ubiquitously express cytoplasmic tdTomato, allowing descendants to be traced after grafting or in cell culture.ConventionalAvailableChameleon (Cytbow)Further informationReporter line. Cre-inducible mini-Brainbow.Ubiquitous expression of nuclear-blueFP, replaced after CRE-mediated recombination by expression of eYFP or tdTomato or mCerulean. ConventionalAvailableLimited supplyCSF1R reporter linesFurther information Macrophage reporter lines – CSF1R-eGFP (MacGreen) and CSF1R-mApple (MacRed).We have demonstrated utility for investigation of immune response in chickens.Used to visualise cells of the mononuclear phagocyte system (MPS; monocytes, macrophages and dendritic cells). ConventionalAvailableLimited supply of CSF1R-mAppleRUNX1-eGFPFurther InformationThe RUNX1-eGFP line can be used to visualise granulocytes (Basophils, Eosinophils and Heterophils) and monocytes. ConventionalAvailableSplit GFPFurther InformationIn the Split GFP line the large subunit of GFP (GFP1-10) only fluoresces when the complementary small subunit of GFP-11 is delivered to a cell. This line is useful for studying infection processes and understanding the development and function of neuromuscular junctions in birds. ConventionalAvailable Cryopreserved LinesGenetically altered linesDescriptionAvailabilityLox-STOP-loxFurther information Reporter line. Inducible expression of eGFP by CRE-mediated recombination, either by CRE protein application or injection of a CRE-expressing plasmid.This allows cells or specific cell populations to be marked and tracked during embryonic development.Only available ascryopreserved materialsNotch reporterFurther informationReporter line used for real-time visualisation of Notch signalling. Only available ascryopreserved materialstalpid3 Further information Talpid mutants are classical recessive embryonic lethal chicken mutants with abnormal limb patterning and malformations. Only available ascryopreserved materialsDDX4 KO Sterile female surrogate host Further information This genetically sterile female surrogate host chicken is used for the creation of new genetically altered poultry lines.Sterile female host birds can transmit transplanted donor primordial germ cells to 100% of their offspring.Only available ascryopreserved materials Please let us know if you want further information on any of these lines or are interested in a collaboration based on our expertise Help us to better understand how you are using eggs, chickens, and our facilities. We welcome feedback from collaborators and new users and use it to guide our research and development of new chicken lines. Contact us Please let us know if you want further information on any of these lines or are interested in a collaboration based on our expertise Help us to better understand how you are using eggs, chickens, and our facilities. We welcome feedback from collaborators and new users and use it to guide our research and development of new chicken lines. Contact us This article was published on 2024-09-02
HTML Fertile eggs and chicks are available for research use upon request, with a requirement for a material transfer agreement for some lines to meet our restrictions relating to animal experiments or the source of the lines.Resources for gene editingGenetically altered linesDescriptionFacilityAvailabilityiCaspase9 surrogate hosts Further informationThe iCaspase9 line can be used to create new genetically altered poultry lines. In the iCaspase9 surrogate host line, both male and female germ cell lineages can be chemically ablated.Sire Dam Surrogate mating results in 100% transmission of introduced primordial germ cells in the first generation of offspring.Conventionaland SPFAvailable Reporter linesGenetically altered lines DescriptionFacilityAvailabilityACTN (actin and nuclear markers)Further information Reporter line. Life-actin-Venus, Histone H2B-cherry. Ubiquitous expression of life-actin-VenusFP and nuclear-cherryFP ConventionalAvailableRoslin Green(Cytoplasmic GFP) Further information Reporter line. Cells from GFP chickens ubiquitously express cytoplasmic eGFP, allowing descendants to be traced after grafting or in cell culture. ConventionalAvailableMembrane GFPFurther informationReporter line. Membrane-localised eGFP.Useful for identifying individual cell boundaries when imaging. ConventionalAvailableFlamingo (TdTomato)Further information Reporter line. Cells from this line ubiquitously express cytoplasmic tdTomato, allowing descendants to be traced after grafting or in cell culture.ConventionalAvailableChameleon (Cytbow)Further informationReporter line. Cre-inducible mini-Brainbow.Ubiquitous expression of nuclear-blueFP, replaced after CRE-mediated recombination by expression of eYFP or tdTomato or mCerulean. ConventionalAvailableLimited supplyCSF1R reporter linesFurther information Macrophage reporter lines – CSF1R-eGFP (MacGreen) and CSF1R-mApple (MacRed).We have demonstrated utility for investigation of immune response in chickens.Used to visualise cells of the mononuclear phagocyte system (MPS; monocytes, macrophages and dendritic cells). ConventionalAvailableLimited supply of CSF1R-mAppleRUNX1-eGFPFurther InformationThe RUNX1-eGFP line can be used to visualise granulocytes (Basophils, Eosinophils and Heterophils) and monocytes. ConventionalAvailableSplit GFPFurther InformationIn the Split GFP line the large subunit of GFP (GFP1-10) only fluoresces when the complementary small subunit of GFP-11 is delivered to a cell. This line is useful for studying infection processes and understanding the development and function of neuromuscular junctions in birds. ConventionalAvailable Cryopreserved LinesGenetically altered linesDescriptionAvailabilityLox-STOP-loxFurther information Reporter line. Inducible expression of eGFP by CRE-mediated recombination, either by CRE protein application or injection of a CRE-expressing plasmid.This allows cells or specific cell populations to be marked and tracked during embryonic development.Only available ascryopreserved materialsNotch reporterFurther informationReporter line used for real-time visualisation of Notch signalling. Only available ascryopreserved materialstalpid3 Further information Talpid mutants are classical recessive embryonic lethal chicken mutants with abnormal limb patterning and malformations. Only available ascryopreserved materialsDDX4 KO Sterile female surrogate host Further information This genetically sterile female surrogate host chicken is used for the creation of new genetically altered poultry lines.Sterile female host birds can transmit transplanted donor primordial germ cells to 100% of their offspring.Only available ascryopreserved materials Please let us know if you want further information on any of these lines or are interested in a collaboration based on our expertise Help us to better understand how you are using eggs, chickens, and our facilities. We welcome feedback from collaborators and new users and use it to guide our research and development of new chicken lines. Contact us
