Laser Scanning Confocal Microscopy

All confocal microscopes require training from designated individuals before use.

Confocal image of macrophages phagocytosing FITC-labelled zymosan (yeast)
Confocal image of macrophages phagocytosing FITC-labelled zymosan (yeast)

Charges apply (see https://booker.is.ed.ac.uk for current charges).

Labs G.064

  • Zeiss LSM 880 Airyscan/Fastscan (7 laser lines, 2PMTs + 32channel Quasar GaAsP detector, Airy/fastscan detector, Tiling/z-stacking/live cell incubation/gas box)
  • Confocal image of macrophages phagocytosing FITC-labelled zymosan (yeast)

Zeiss Elyra 7 with Lattice SIM²

  • Reveals molecular architecture at nanometre precision to explore fine sub-cellular detail.
  • Fast super-resolution and single-molecule localisation imaging
  • Dual sCMOS cameras (Hamamatsu ORCA-Fusion BT) — up to 255 fps
  • 4 laser (405, 488, 561, 640nm)
  • Resolution down to 60 nm lateral (live or fixed)
  • Supports DNA-PAINT, dSTORM and PALM (10–20 nm precision)
Zeiss Elyra 7 with Lattice SIM²