Expertise

In addition to all standard molecular biology techniques, members of the RICE group have expertise in a number of procedures.

1. In ovo manipulation of chicken embryos

  • bead implantation
  • microdissection
  • cell and tissue grafting
  • cell and tissue transplants
  • application of growth factors and pathway inhibitors
  • siRNA knock-down
  • Antagomir suppression
  • in ovo electroporation
  • lenti virus expression

2. Generation of transgenic birds

  • Lentivirus injection
  • PGC editing

3. Primordial Germ Cell (PGC) culture

4. Chicken embryo fibroblast (CEF) isolation and culture

5. Organ culture

  • Skin
  • Gonad

6. Embryo culture

  • ‘New’ Technique
  • EC (easy) Technique

https://link.springer.com/protocol/10.1007%2F978-1-60327-483-8_17#page

7. Microscopy

  • Super resolution microscopy
  • Electron microscopy
  • Confocal microscopy
  • Optical projection Tomography
  • Time-lapse

8. Lenti-virus production

9. Immunohistochemistry

10. Whole mount RNA in situ hybridisation

11. Sequencing from ancient and fixed tissues

12. In vitro CRISPR gene editing

13. miRNA analysis

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