In addition to all standard molecular biology techniques, members of the RICE group have expertise in a number of procedures. 1. In ovo manipulation of chicken embryos bead implantation microdissection cell and tissue grafting cell and tissue transplants application of growth factors and pathway inhibitors siRNA knock-down Antagomir suppression in ovo electroporation lenti virus expression 2. Generation of transgenic birds Lentivirus injection PGC editing 3. Primordial Germ Cell (PGC) culture 4. Chicken embryo fibroblast (CEF) isolation and culture 5. Organ culture Skin Gonad 6. Embryo culture ‘New’ Technique EC (easy) Technique https://link.springer.com/protocol/10.1007%2F978-1-60327-483-8_17#page 7. Microscopy Super resolution microscopy Electron microscopy Confocal microscopy Optical projection Tomography Time-lapse 8. Lenti-virus production 9. Immunohistochemistry 10. Whole mount RNA in situ hybridisation 11. Sequencing from ancient and fixed tissues 12. In vitro CRISPR gene editing 13. miRNA analysis This article was published on 2024-09-02
