The NARF has gene editing facilities for the chicken, a variety of reporter lines and a natural mutation line. Fertile eggs and chicks are available for research use upon request, with a requirement for a material transfer agreement for some lines to meet our restrictions relating to animal experiments or the source of the lines. Resources for gene editing Genetically altered lines Description Facility Availability iCaspase9 surrogate hosts Further information The iCaspase9 line can be used to create new genetically altered poultry lines. In the iCaspase9 surrogate host line, both male and female germ cell lineages can be chemically ablated. Sire Dam Surrogate mating results in 100% transmission of introduced primordial germ cells in the first generation of offspring. Conventional and SPF Available Reporter lines Genetically altered lines Description Facility Availability ACTN (actin and nuclear markers) Further information Reporter line. Life-actin-Venus, Histone H2B-cherry. Ubiquitous expression of life-actin-VenusFP and nuclear-cherryFP Conventional Available Roslin Green (Cytoplasmic GFP) Further information Reporter line. Cells from GFP chickens ubiquitously express cytoplasmic eGFP, allowing descendants to be traced after grafting or in cell culture. Conventional Available Membrane GFP Further information Reporter line. Membrane-localised eGFP. Useful for identifying individual cell boundaries when imaging. Conventional Available Flamingo (TdTomato) Further information Reporter line. Cells from this line ubiquitously express cytoplasmic tdTomato, allowing descendants to be traced after grafting or in cell culture. Conventional Available Chameleon (Cytbow) Further information Reporter line. Cre-inducible mini-Brainbow. Ubiquitous expression of nuclear-blueFP, replaced after CRE-mediated recombination by expression of eYFP or tdTomato or mCerulean. Conventional Available Limited supply CSF1R reporter lines Further information Macrophage reporter lines – CSF1R-eGFP (MacGreen) and CSF1R-mApple (MacRed). We have demonstrated utility for investigation of immune response in chickens. Used to visualise cells of the mononuclear phagocyte system (MPS; monocytes, macrophages and dendritic cells). Conventional Available Limited supply of CSF1R-mApple RUNX1-eGFP Further Information The RUNX1-eGFP line can be used to visualise granulocytes (Basophils, Eosinophils and Heterophils) and monocytes. Conventional Available Split GFP Further Information In the Split GFP line the large subunit of GFP (GFP1-10) only fluoresces when the complementary small subunit of GFP-11 is delivered to a cell. This line is useful for studying infection processes and understanding the development and function of neuromuscular junctions in birds. Conventional Available Cryopreserved Lines Genetically altered lines Description Availability Lox-STOP-lox Further information Reporter line. Inducible expression of eGFP by CRE-mediated recombination, either by CRE protein application or injection of a CRE-expressing plasmid. This allows cells or specific cell populations to be marked and tracked during embryonic development. Only available as cryopreserved materials Notch reporter Further information Reporter line used for real-time visualisation of Notch signalling. Only available as cryopreserved materials talpid3 Further information Talpid mutants are classical recessive embryonic lethal chicken mutants with abnormal limb patterning and malformations. Only available as cryopreserved materials DDX4 KO Sterile female surrogate host Further information This genetically sterile female surrogate host chicken is used for the creation of new genetically altered poultry lines. Sterile female host birds can transmit transplanted donor primordial germ cells to 100% of their offspring. Only available as cryopreserved materials HTML Please let us know if you want further information on any of these lines or are interested in a collaboration based on our expertise Help us to better understand how you are using eggs, chickens, and our facilities. We welcome feedback from collaborators and new users and use it to guide our research and development of new chicken lines. Contact us This article was published on 2024-09-02
HTML Please let us know if you want further information on any of these lines or are interested in a collaboration based on our expertise Help us to better understand how you are using eggs, chickens, and our facilities. We welcome feedback from collaborators and new users and use it to guide our research and development of new chicken lines. Contact us
