A full day, hands-on workshop working with proteins and antibodies. A chance for your AH pupils to work with our scientists and use real lab equipment. In this enzyme-linked immunosorbent assay (ELISA) masterclass, your pupils will diagnose the disease status of chickens to identify which are infected with the influenza virus. Learning Level: S6 Advanced Higher Location: Easter Bush Science Outreach Centre, University of Edinburgh Easter Bush Campus, EH25 9RGMinimum attendance: 16 ( If your numbers are below 16, please let us know and we will do our best to twin you with another group).Maximum attendance: 24Duration: 5 hoursTimes: 10am - 3pmCost: £10 per pupil (if this is a barrier to attending, please email ebsoc@ed.ac.uk in confidence)Availability: 2025 dates to be announcedI thought the workshop was planned really well, ran really smoothly and was well thought through. Would definitely recommend this workshop as I think it’s a great experience for a young person interested in pursuing science. Thank you for the opportunity :)Advanced Higher Biology Student - St Joseph's College, Dumfries Learning ObjectivesUnderstand the structure, lifecycle and modes of transmission of avian fluUnderstand how ELISA can be used to detect influenza infectionWill do a quantitative ELISA of flu antibody levels to detect signs of infectionMake a dilution series and create a standard curve of known antigen concentrationsUse micropipettes and microplate readerInterpretation of experimental resultsTo reveal the world of work in scientific research Techniques UsedELISA - working with proteins and antibodiesQuantification of protein concentrations- using a microplate readerCentrifugationMicro-pipettingWorkshop outlineELISA jigsaw activity to teach/reinforce the ELISA techniqueIntroduction to influenza virus and avian fluIntroduction to micropipettesSerial dilution to generate dilutions of known antigen concentration.Quantitative ELISA to assay infection status of several chickensAnalysis and interpretation of resultsDiscussion with scientists from The Roslin Institute Curriculum linksCells and ProteinsOrganisms and EvolutionInvestigative Biology1 Laboratory Techniques:Health and SafetyLiquids and SolutionsAntibody TechniquesMicroscopy 5 Parasitism:The parasite nicheTransmission and virulenceImmune response to parasitesParasitic life cyclesChallenges in treatment and control1 Scientific principles and process:Scientific ethics 2 Experimentation:ControlsEnsuring reliabilityDeveloping the Young Workforce – “I can” statements The following "I can" statements are supported through EBSOC’s workshops: I can identify the skills I have learnt across the curriculum, how these relate to the world of work and can apply these appropriately during work placements and other work-related learning.I can confidently access and interpret the information I need to make well informed choices about my learning options, pathways and how these relate to possible future careers.I can work towards achieving qualifications which support me to achieve my future career aspirations.AvailabilityMore details about our secondary programme will be released shortly, to remain updated about our workshops, events and exciting opportunities please join our mailing list by clicking here. Important Notes* Please contact us ebsoc@ed.ac.uk if your group falls below the minimum as we may be able to accommodate your request through our small groups waiting list. Downloadable Resources Document Elisa Masterclass Risk Assessement (616.88 KB / PDF) Document ELISA Masterclass: Flu Fighters Risk Assessement (615.82 KB / PDF) TestimonialsTalking to actual scientists was very interesting.Higher Biology PupilCould work with people who are doing current, cutting edge research. We also got to use more advanced equipment than in school.Higher Biology PupilThe workshop put the ELISA into context for the student and was very hands on for them I thought the chat to a scientist time at the end was great to show students the variety of options available when studying/working in scienceSecondary Biology TeacherIt provided the pupils with hands-on experience, provided them data to process and the implications of that data in the real world. Secondary Biology Teacher ELISA Masterclass This article was published on 2024-09-02