A full day, hands-on workshop working with proteins and antibodies. A chance for your AH pupils to work with our scientists and use real lab equipment. In this enzyme-linked immunosorbent assay (ELISA) masterclass, your pupils will diagnose the disease status of chickens to identify which are infected with the influenza virus. Learning Level: S6 Advanced Higher Location: Easter Bush Science Outreach Centre, University of Edinburgh Easter Bush Campus, EH25 9RG Minimum attendance: 16 ( If your numbers are below 16, please let us know and we will do our best to twin you with another group). Maximum attendance: 24 Duration: 5 hours Times: 10am - 3pm Cost: £10 per pupil (if this is a barrier to attending, please email ebsoc@ed.ac.uk in confidence) Availability: November 19, 20, 21, 26 & 27 December 3 & 5 Booking enquiry forms close Friday 30 August. Please note a booking enquiry form is not a booking. We try our best to accommodate everyone but these workshops are very popular. Proteins & Antibodies ELISA Masterclass: Flu Fighters - Booking Enquiry Form I thought the workshop was planned really well, ran really smoothly and was well thought through. Would definitely recommend this workshop as I think it’s a great experience for a young person interested in pursuing science. Thank you for the opportunity :) Advanced Higher Biology Student - St Joseph's College, Dumfries Learning Objectives Understand the structure, lifecycle and modes of transmission of avian flu Understand how ELISA can be used to detect influenza infection Will do a quantitative ELISA of flu antibody levels to detect signs of infection Make a dilution series and create a standard curve of known antigen concentrations Use micropipettes and microplate reader Interpretation of experimental results To reveal the world of work in scientific research Techniques Used ELISA - working with proteins and antibodies Quantification of protein concentrations- using a microplate reader Centrifugation Micro-pipetting Workshop outline ELISA jigsaw activity to teach/reinforce the ELISA technique Introduction to influenza virus and avian flu Introduction to micropipettes Serial dilution to generate dilutions of known antigen concentration. Quantitative ELISA to assay infection status of several chickens Analysis and interpretation of results Discussion with scientists from The Roslin Institute Curriculum links Cells and Proteins Organisms and Evolution Investigative Biology 1 Laboratory Techniques: Health and Safety Liquids and Solutions Antibody Techniques Microscopy 5 Parasitism: The parasite niche Transmission and virulence Immune response to parasites Parasitic life cycles Challenges in treatment and control 1 Scientific principles and process: Scientific ethics 2 Experimentation: Controls Ensuring reliability Developing the Young Workforce – “I can” statements The following "I can" statements are supported through EBSOC’s workshops: I can identify the skills I have learnt across the curriculum, how these relate to the world of work and can apply these appropriately during work placements and other work-related learning. I can confidently access and interpret the information I need to make well informed choices about my learning options, pathways and how these relate to possible future careers. I can work towards achieving qualifications which support me to achieve my future career aspirations. Availability More details about our secondary programme will be released shortly, to remain updated about our workshops, events and exciting opportunities please join our mailing list by clicking here. Important Notes * Please contact us ebsoc@ed.ac.uk if your group falls below the minimum as we may be able to accommodate your request through our small groups waiting list. Downloadable Resources Risk Assessment Document ELISA Masterclass Risk Assessment (616.88 KB / PDF) Workshop Description Document ELISA Flu Fighters Secondary Workshop Description July 2018 (574.49 KB / PDF) Testimonials Talking to actual scientists was very interesting. Higher Biology Pupil Could work with people who are doing current, cutting edge research. We also got to use more advanced equipment than in school. Higher Biology Pupil The workshop put the ELISA into context for the student and was very hands on for them I thought the chat to a scientist time at the end was great to show students the variety of options available when studying/working in science Secondary Biology Teacher It provided the pupils with hands-on experience, provided them data to process and the implications of that data in the real world. Secondary Biology Teacher This article was published on 2024-09-02
