Developing methods to propagate macrophages from stem cells and using those macrophages to investigate interactions with Mycobacterium bovis - the causative agent of bovine TB. This is a BBSRC EASTBIO studentship funded for four years from October 2021 to September 2025. With Kishan Dholakia at the University of St Andrews, we will use specialised imaging techniques to understand the intracellular life of M. bovis and how it evades destruction within the macrophage. Team members: Emily Charlton Team partners: Tom Burdon and Stephen Meek at Roslin; Kishan Dholakia at the University of St Andrews Key references Baron, V. O., Chen, M., Clark, S. O., Williams, A., Dholakia, K., & Gillespie, S. H. (2018). Detecting Phenotypically Resistant Mycobacterium tuberculosis Using Wavelength Modulated Raman Spectroscopy. Methods in molecular biology (Clifton, N.J.), 1736, 41–50. https://doi.org/10.1007/978-1-4939-7638-6_4 Meek, S., Wei, J., Oh, T., Watson, T., Olavarrieta, J., Sutherland, L., Carlson, D. F., Salzano, A., Chandra, T., Joshi, A., & Burdon, T. (2020). A Stem Cell Reporter for Investigating Pluripotency and Self-Renewal in the Rat. Stem cell reports, 14(1), 154–166. https://doi.org/10.1016/j.stemcr.2019.12.001 This article was published on 2024-09-02
